A novel immobilization strategy for electrochemical detection of cancer biomarkers: DNA-directed immobilization of aptamer sensors for sensitive detection of prostate specific antigens.
نویسندگان
چکیده
We report on a novel strategy for DNA aptamer immobilization to develop sensitive electrochemical detection of a protein biomarker, with prostate specific antigen (PSA) as a case biomarker. Thiolated single-stranded DNA (ssDNA) was co-immobilized with 3-mercapto-1-propanol on gold electrodes, and used as a scaffold for DNA aptamer attachment through hybridization of the aptamer overhang (so-called "DNA-directed immobilization aptamer sensors", DDIAS). In the approach, the complementary DNA aptamer against PSA was assembled by the probe ssDNA onto the electrode to detect PSA; or the probe ssDNA directly hybridized with a complementary DNA aptamer/PSA complex following their pre-incubation in solution, so-called 'on-chip' and 'in-solution' methods, respectively. A double stranded DNA intercalator with a ferrocenyl (Fc) redox marker was synthesized to evaluate the feasibility of the strategy. The results demonstrate that the 'in-solution' method offers a favourable medium (in a homogeneous solution) for the binding between the aptamer and PSA, which shows to be more efficient than the 'on-chip' approach. DDIAS shows promising analytical performance under optimized conditions, with a limit of detection in the range of fM and low non-specific adsorption.
منابع مشابه
A novel immobilization strategy for electrochemical detection of cancer biomarkers: DNA-directed immobilization of aptamer sensors for sensitive detection of prostate specific antigen†
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تشخیص آنتی‌ژن اختصاصی پروستات با استفاده از بیوسنسور الکتروشیمیایی مبتنی بر آپتامر
Background and Objectives: Detection of the biomarkers is one of the effective methods for diagnosis and treatment of prostate cancer. Prostate specific antigen (PSA) is currently the best biomarker available for controlling and detecting this cancer. The purpose of the current study was to design an electrochemical aptamer-based biosensor (electrochemical aptasensor) to measure...
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ورودعنوان ژورنال:
- The Analyst
دوره 140 8 شماره
صفحات -
تاریخ انتشار 2015